Continuous Liquid-Liquid Partition Chromatography

  • Low cost of operation
    (No solid stationary phase)
  • Ease of process development
  • Scalable from bench to plant

Description

Zaiput membrane based separators enable, with appropriate configuration, continuous separation of chemical species leveraging the principle of Partition Chromatography.

When Crystallization isn’t an option, CLL-PC provide a cost effective solution to separate chemical species at a fraction of the cost of Simulated moving bed Chromatography.

Our method and related hardware offer the ability to carry out partition chromatography  in a continuous manner. In our approach phase separation is accomplished with the Zaiput membrane  based devices.

 An adequately designed multistage extraction process with >5 stages allows to retrieve a phase with one species ( or one group of species) and another phase  with the other species ( or another group of species). In other words, very much like simulated moving bed chromatography the technique allows to separate a continuous feed into two stream each one with a distinct group of species in it. 

What is partition Chromatography? 

The concepts of Partition Chromatography have been developed in the 40s, a Nobel price in Chemistry was awarded to Martin and Since in 1952 for this discovery. 

Very much like solid stationary phase chromatography where the travel speed of analytes is altered by interaction with a solid stationary phase thus achieving separation, in partition chromatography ( also known as liquid-liquid chromatography) the travel on analytes is altered by interaction (partitioning) with a second phase that, in this case, is also a liquid.

 The challenge of this approach has always been its practical implementation Several approaches have been presented over the decades. All of them have high complexity of the mechanical apparatus involved. More recently the use of centrifugal force ( Centrifugal partition chromatography , CPC) has gained some popularity despite the challenges of associated high pressure in rotating systems.

How It works

An adequately designed countercurrent multistage extraction process with >5 stages allows to retrieve a phase with one species ( or one group of species) and another phase  with the other species ( or another group of species). In other words, very much like simulated moving bed chromatography the technique allows to separate a continuous feed into two stream each one with a distinct group of species in it.

The process design requirement for a countercurrent LLE process to provide a continuous partition chromatography type of separation is to ensure that the species to separate have one partition coefficient greater than 1 and the other lower than 1. The greater is the difference between the partition coefficients the lower is the number of stages needed.

Applications

  • Separation of Chiral Molecules 
  • Separation of Natural products
  • Separation of Isomers

CLL-PC allows separation of chemical specie leveraging differences in partitioning of the species not two different liquid phases.  Applications are across a wide spectrum, in the literature partition chromatography has been applied in several areas such as separation of chiral molecules, natural products , isomers in general.

The Zaiput unique approach to continuous  Partition Chromatography has two key features :

  • No need of density difference between the liquid phases, thus expanding application space
  • No moving parts, thus providing robust industrial solution

Development of an application

To develop an application there are 3 key steps to follow iteratively until adequate performance is achieved:

  1. Identify one or more solvent systems that provide differences in partitioning of the chemical  species to separate. 
  2. With the assistance of a Zaiput representative, using our model of the continuous partition chromatography process,  verify outcome.
  3. Carry out experimental test on our laboratory scale platform
  4. Iterate process steps 1,2,3 (solvent system optimization, model and experimental results) until process specification/ goals are demonstrated experimentally.
  5. Scale up to desired scale with Zaiput larger scale devices. 

Literature

Viano, R., Adamo, A., et al., 2023. Organic Process Research & Development, 27(12), pp.2296-2308.

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